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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 104, No. 5, 2009, pp. 736-744
Bioline Code: oc09164
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 104, No. 5, 2009, pp. 736-744

 en Infection kinetics of human adenovirus serotype 41 in HEK 293 cells
Siqueira-Silva, Joselma; Yeda, Fernanda Perez; Favier, Anne-Laure; Mezin, Paulette; Silva, Misael Leonardo; Barrella, Karina Médici; Mehnert, Dolores Úrsula; Fender, Pascal & Hársi, Charlotte Marianna

Abstract

The purpose of this work was to acquire an overview of the infectious cycle of HAdV-41 in permissive HEK 293 cells and compare it to that observed with the prototype of the genus, Human adenovirus C HAdV-2. HEK 293 cells were infected with each virus separately and were harvested every 12 h for seven days. Infection kinetics were analysed using confocal and electronic microscopy. The results show that, when properly cultivated, HAdV-41 was not fastidious. It had a longer multiplication cycle, which resulted in the release of complete viral particles and viral stocks reached high titres. After 60 h of infection, the export of viral proteins from the infected cell to the extracellular milieu was observed, with a pattern similar to that previously described for HAdV-2 penton-base trafficking after 30 h of infection. HAdV-41 had a non-lytic cycle and the infection spread from the first infected cell to its neighbours. The release process of the viral particles is unknown. The results observed for HAdV-41 infection in HEK 293 cells show how different this virus is from the prototype HAdV-2 and provides information for the development of this vector for use in gene therapy.

Keywords
Human adenovirus F - infection kinetics - virus cultivation - confocal microscopy - electronic microscopy

 
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