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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 104, No. 5, 2009, pp. 745-748
Bioline Code: oc09165
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 104, No. 5, 2009, pp. 745-748

 en Molecular and biochemical characterisation of Trypanosoma cruzi check for this species in other resources phosphofructokinase
Rodríguez, Evelyn; Lander, Noelia & Ramirez, Jose Luis

Abstract

The characterisation of the gene encoding Trypanosoma cruzi check for this species in other resources CL Brener phosphofructokinase (PFK) and the biochemical properties of the expressed enzyme are reported here. In contradiction with previous reports, the PFK genes of CL Brener and YBM strain T. cruzi were found to be similar to their Leishmania mexicana check for this species in other resources and Trypanosoma brucei check for this species in other resources homologs in terms of both kinetic properties and size, with open reading frames encoding polypeptides with a deduced molecular mass of 53,483. The predicted amino acid sequence contains the C-terminal glycosome-targeting tripeptide SKL; this localisation was confirmed by immunofluorescence assays. In sequence comparisons with the genes of other eukaryotes, it was found that, despite being an adenosine triphosphate-dependent enzyme, T. cruzi PFK shows significant sequence similarity with inorganic pyrophosphate-dependent PFKs.

Keywords
Trypanosoma cruzi - Kinetoplastida - phosphofructokinase - glycolytic enzyme - molecular biochemical characterisation

 
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