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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 104, No. 5, 2009, pp. 769-774
Bioline Code: oc09169
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 104, No. 5, 2009, pp. 769-774

 en Molecular characterisation of Sporothrix schenckii check for this species in other resources isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil
Reis, Rosani Santos; Almeida-Paes, Rodrigo; Muniz, Mauro de Medeiros; Tavares, Patrícia Morais e Silva; Monteiro, Paulo Cezar Fialho; Schubach, Tânia Maria Pacheco; Gutierrez-Galhardo, Maria Clara & Zancopé-Oliveira, Rosely Maria

Abstract

An epidemic of sporotrichosis, a subcutaneous mycosis caused by the fungus Sporothrix schenckii check for this species in other resources , is ongoing in Rio de Janeiro, Brazil, in which cases of human infection are related to exposure to cats. In an attempt to demonstrate the zoonotic character of this epidemic using molecular methodology, we characterised by DNA-based typing methods 19 human and 25 animal S. schenckii isolates from the epidemic, as well as two control strains. To analyse the isolates, the random amplified polymorphic DNA (RAPD) technique was performed using three different primers, together with DNA fingerprinting using the minisatellite derived from the wild-type phage M13 core-sequence. The analyses generated amplicons with considerable polymorphism. Although isolates exhibited high levels of genetic relatedness, they could be clustered into 5-10 genotypes. The RAPD profiles of epidemic S. schenckii isolates could be distinguished from that of the United States isolate, displaying 20% similarity to each primer and 60% when amplified with the M13 primer. DNA fingerprinting of S. schenckii isolated from the nails (42.8%) and the oral cavities (66%) of cats were identical to related human samples, suggesting that there is a common infection source for animals and humans in this epidemic. It is clear that cats act as a vehicle for dissemination of S. schenckii.

Keywords
Sporotrichosis - zoonotic transmission - RAPD - DNA fingerprinting

 
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