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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 104, No. 8, 2009, pp. 1055-1062
Bioline Code: oc09219
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 104, No. 8, 2009, pp. 1055-1062

 en Inhibition of Trypanosoma cruzi check for this species in other resources proline racemase affects host-parasite interactions and the outcome of in vitro infection
Coutinho, Leticia; Ferreira, Marcelo Alves; Cosson, Alain; Batista, Marcos Meuser; Batista, Denise da Gama Jaén; Minoprio, Paola; Degrave, Wim M; Berneman, Armand & Soeiro, Maria de Nazaré Correia

Abstract

Proline racemase is an important enzyme of Trypanosoma cruzi check for this species in other resources and has been shown to be an effective mitogen for B cells, thus contributing to the parasite’s immune evasion and persistence in the human host. Recombinant epimastigote parasites overexpressing TcPRAC genes coding for proline racemase present an augmented ability to differentiate into metacyclic infective forms and subsequently penetrate host-cells in vitro. Here we demonstrate that both anti T. cruzi proline racemase antibodies and the specific proline racemase inhibitor pyrrole-2- carboxylic acid significantly affect parasite infection of Vero cells in vitro. This inhibitor also hampers T. cruzi intracellular differentiation.

Keywords
Trypanosoma cruzi - proline racemase - host-parasite interaction - enzyme inhibition

 
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