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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 1, 2010, pp. 62-65
Bioline Code: oc10009
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 1, 2010, pp. 62-65

 en Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis check for this species in other resources
Batista, João Júnior; Berzaghi, Rodrigo; Arnaud, Adelita Duanra Medeiros do Monte; Fontes, Cor Jesus Fernandes; de Camargo, Zoilo Pires & Hahn, Rosane Christine

Abstract

This study is the first report on genetic differences between isolates of Paracoccidioides brasiliensis check for this species in other resources from a single patient. We describe a simultaneous infection with genetically distinct isolates of P. brasiliensis in a patient with chronic paracoccidioidomycosis. The clinical isolates were obtained from lesions in different anatomical sites and were characterised by random amplified polymorphic DNA (RAPD) analysis. The RAPD technique can be helpful for distinguishing between clinical isolates. Different random primers were used to characterise these clinical isolates. The RAPD patterns allowed for differentiation between isolates and the construction of a phenetic tree, which showed more than 28% genetic variability in this fungal species, opening new possibilities for clinical studies of P. brasiliensis. Based on these results and preliminary clinical findings, we suggest that different genotypes of P. brasiliensis might infect the same patient, inducing the active form of the disease.

Keywords
Paracoccidioides brasiliensis - simultaneous infection - RAPD

 
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