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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 105, No. 1, 2010, pp. 109-112
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Bioline Code: oc10018
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 1, 2010, pp. 109-112
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Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre
Fagundes, Aline; Schubach, Armando; de Paula, Cíntia Cristiane; Bogio, Alessandra; Antonio, Liliane de Fátima; Schiavoni, Patrícia Botelho; Monteiro, Vivian de Souza; Madeira, Maria de Fátima; Quintella, Leonardo Pereira; Valete-Rosalino, Claudia Maria; Ferreira e Vasconcellos, Erica de Camargo; de Azeredo-Coutinho, Rilza Beatriz Gayoso; Pacheco, Rachel S; Marzochi, Mauro CA & Marzochi, Keyla BF
Abstract
The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel
to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy
parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established
with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF
group). PCR showed 92.3% sensitivity, 93.3% specificity, a 99.2% positive predictive value and a 13.84 positive
likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis
treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine
diagnostic procedures for ATL, especially for cases found to be negative by conventional methods.
Keywords
Leishmania - polymerase chain reaction - diagnosis
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