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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 2, 2010, pp. 184-190
Bioline Code: oc10032
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 2, 2010, pp. 184-190

 en Chemical composition and antiprotozoal activities of Colombian Lippia check for this species in other resources essential oils and their major components
Escobar, Patricia; Leal, Sandra Milena; Herrera, Laura Viviana; Martinez, Jairo Rene & Stashenko, Elena

Abstract

The chemical composition and biological activities of 19 essential oils and seven of their major components were tested against free and intracellular forms of Leishmania chagasi check for this species in other resources and Trypanosoma cruzi check for this species in other resources parasites as well as Vero and THP-1 mammalian cell lines. The essential oils were obtained from different species of Lippia check for this species in other resources , a widely distributed genus of Colombian plants. They were extracted by microwave radiation-assisted hydro-distillation and characterised by GC-FID and GC-MS. The major components were geranial, neral, limonene, nerol, carvacrol, p-cymene, γ-terpinene, carvone and thymol. The essential oil of Lippia alba check for this species in other resources exhibited the highest activity against T. cruzi epimastigotes and intracellular amastigotes with an IC50 of 5.5 μg/mL and 12.2 μg/mL, respectively. The essential oil of Lippia origanoides check for this species in other resources had an IC50 of 4.4 μg/mL, in L. chagasi promastigotes and exhibited no toxicity in mammalian cells. Thymol (IC50 3.2 ± 0.4 μg/mL) and S-carvone (IC50 6.1 ± 2.2 μg/mL), two of the major components of the active essential oils, were active on intracellular amastigotes of T. cruzi infected Vero cells, with a selective index greater than 10. None of the essential oils or major components tested in this study was active on amastigotes of L. chagasi infected THP-1 cells.

Keywords
essential oils, Lippia, cytotoxic activity, Leishmania chagasi, Trypanosoma cruzi, Colombia

 
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