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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 105, No. 3, 2010, pp. 269-277
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Bioline Code: oc10046
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 3, 2010, pp. 269-277
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Vaccinia virus regulates expression of p21WAF1/Cip1 in A431 cells
Andrade, Anderson A.; Brasil, Bruno S.A.F.; Pereira, Anna C.T.C.; Ferreira, Paulo C.P.; Kroon, Erna G. & Bonjardim, Cláudio A.
Abstract
In this paper, we provide evidence that both the mRNA and protein levels of the cyclin-dependent kinase (CDK) inhibitor
p21WAF1/CDK-interacting protein 1 (Cip1) increase upon infection of A431 cells with Vaccinia virus (VACV). In addition,
the VACV growth factor (VGF) seems to be required for the gene expression because infection carried out with the mutant
virus VACV-VGF- revealed that this strain was unable to stimulate its transcription. Our findings are also consistent
with the notion that the VGF-mediated change in p21WAF1/Cip1 expression is dependent on tyrosine kinase pathway(s)
and is partially dependent on mitogen-activated protein kinase/extracellular-signal regulated kinase 1/2. We believe
that these pathways are biologically significant because VACV replication and dissemination
was drastically affected when the infection was carried out in the presence of the relevant pharmacological inhibitors.
Keywords
Vaccinia virus, virus-host cell interaction, p21WAF1/Cip1, MEK/ERK1/2, signal transduction
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