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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 3, 2010, pp. 310-313
Bioline Code: oc10052
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 3, 2010, pp. 310-313

 en Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children
Fraga, Thiago Leite; Brustoloni, Yvone Maia; Lima, Rosimar Baptista; Dorval, Maria Elizabeth Cavalheiros; Oshiro, Elisa Teruya; Oliveira, Janaina; de Oliveira, Ana Lúcia Lyrio & Pirmez, Claude

Abstract

The diagnosis of visceral leishmaniasis (VL) generally requires the use of invasive tests for the collection of infected tissue (aspirates of bone marrow, spleen, liver, or lymph nodes). This difficulty has led to the search for safer and less painful techniques to confirm the occurence of the disease in children. Polymerase chain reaction (PCR) is a method that is advantageous in that it allows the use of peripheral blood samples for diagnosis. This paper reports the utilization of PCR on peripheral blood samples to diagnose VL in 45 children in Mato Grosso do Sul, Brazil. This technique is compared with methods carried out using tissue collected by invasive procedures, including direct microscopy, culture and detection of Leishmania check for this species in other resources DNA by PCR in bone marrow aspirates. The results show that PCR of peripheral blood provides great sensitivity (95.6%) that is similar to that from the PCR of bone marrow aspirates (91.1%) and higher than that achieved with microscopy (80%) or culture (26.7%) methods. PCR of peripheral blood proved to be a suitable tool for the diagnosis of VL in children because it is highly sensitive and safe, with tissue collection being less invasive than in traditional tests.

Keywords
leishmaniasis, PCR, peripheral blood, children

 
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