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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 3, 2010, pp. 318-321
Bioline Code: oc10054
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 3, 2010, pp. 318-321

 en Biochemical properties, enterohaemolysin production and plasmid carriage of Shiga toxin-producing Escherichia coli check for this species in other resources strains
Souza, Mario R.S.R.S.M.; Klassen, Giseli; De Toni, Fabiana; Rigo, Liu U.; Henkes, Caroline; Pigatto, Caroline P.; Dalagassa, Cibelle de Borba & Fadel-Picheth, Cyntia M.T.

Abstract

Thirty-eight strains of Shiga toxin-producing Escherichia coli check for this species in other resources (STEC) were characterised in terms of biochemical properties, enterohaemolysin production and plasmid carriage. A wide variation in the biochemical properties was observed among the STEC, with 14 distinct biotypes identified. Biotype 1 was the most common, found in 29% of the strains. Enterohaemolysin production was detected in 29% of the strains. Most of the bacterial strains (95%) carried one or more plasmids and considerable heterogeneity in size and combinations was observed. Seven distinct plasmid profiles were identified. The most common profile, characterised by the presence of a single plasmid of ~90 kb, was found in 50% of these strains. These data indicate extensive diversity among STEC strains. No correlation was found among biotype, serotype, enterohaemolysin production and plasmid profile.

Keywords
Escherichia coli, STEC, plasmid profile, enterohaemolysin, biochemical properties

 
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