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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 3, 2010, pp. 341-347
Bioline Code: oc10059
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 3, 2010, pp. 341-347

 en Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis check for this species in other resources and its surface expression increases during metacyclogenesis
Matos, Denise C.S.; Faccioli, Lanuza A.P.; Cysne-Finkelstein, Léa; De Luca, Paula Mello; Corte-Real, Suzana; Armôa, Geraldo R.G.; Lemes, Elezer Monte Blanco; Decote-Ricardo, Débora & Mendonça, Sergio C.F.

Abstract

Kinetoplastid membrane protein-11 (KMP-11), a protein present in all kinetoplastid protozoa, is considered a potential candidate for a leishmaniasis vaccine. A suitable leishmaniasis vaccine candidate molecule must be expressed in amastigotes, the infective stage for mammals. However, the expression of KMP-11 in Leishmania check for this species in other resources amastigotes has been a subject of controversy. We evaluated the expression of this molecule in logarithmic and stationary growth phase promastigotes, as well as in amastigotes, of Leishmania amazonensis check for this species in other resources by immunoblotting, flow cytometry and immunocytochemistry, using a monoclonal antibody against KMP-11. We found that KMP-11 is present in promastigotes and amastigotes. In both stages, the protein was found in association with membrane structures (at the cell surface, flagellar pocket and intracellular vesicles). More importantly, its surface expression is higher in amastigotes than in promastigotes and increases during metacyclogenesis. The increased expression of KMP-11 in metacyclic promastigotes, and especially in amastigotes, indicates a role for this molecule in the parasite relationship with the mammalian host. The presence of this molecule in amastigotes is consistent with the previously demonstrated immunoprotective capacity of vaccine prototypes based on the KMP-11-coding gene and the presence of humoral and cellular immune responses to KMP-11 in Leishmania-infected humans and animals.

Keywords
Leishmania amazonensis, KMP-11, expression, amastigote, promastigote, metacyclogenesis

 
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