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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 3, 2010, pp. 353-355
Bioline Code: oc10061
Full paper language: English
Document type: Note
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 3, 2010, pp. 353-355

 en Multiplex PCR-based detection of Leptospira check for this species in other resources in environmental water samples obtained from a slum settlement
Vital-Brazil, Juliana Magalhães; Balassiano, Ilana Teruszkin; de Oliveira, Fabiano Sutter; Costa, Alberto Dias de Souza; Hillen, Leandro & Pereira, Martha Maria

Abstract

The aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 analysed samples were positive in the multiplex PCR, two were considered to have saprophytic leptospires and one had pathogenic leptospires. The results obtained supported the idea that multiplex PCR can be used to detect Leptospira check for this species in other resources spp in water samples. This method was also able to differentiate between saprophytic and pathogenic leptospires and was able to do so much more easily than conventional methodologies.

Keywords
Leptospira spp, multiplex PCR, water sample

 
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