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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 4, 2010, pp. 391-397
Bioline Code: oc10069
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 4, 2010, pp. 391-397

 en CA88, a nuclear repetitive DNA sequence identified in Schistosoma mansoni check for this species in other resources , aids in the genotyping of nine Schistosoma species of medical and veterinary importance
Bahia, Diana; Rodrigues, Nilton B.; Araújo, Flávio Marcos G.; Romanha, Álvaro José; Ruiz, Jerônimo C.; Johnston, David A. & Oliveira, Guilherme


CA88 is the first long nuclear repetitive DNA sequence identified in the blood fluke, Schistosoma mansoni check for this species in other resources . The assembled S. mansoni sequence, which contains the CA88 repeat, has 8,887 nucleotides and at least three repeat units of approximately 360 bp. In addition, CA88 also possesses an internal CA microsatellite, identified as SmBr18. Both PCR and BLAST analysis have been used to analyse and confirm the CA88 sequence in other S. mansoni sequences in the public database. PCR-acquired nuclear repetitive DNA sequence profiles from nine Schistosoma species were used to classify this organism into four genotypes. Included among the nine species analysed were five sequences of both African and Asian lineages that are known to infect humans. Within these genotypes, three of them refer to recognised species groups. A panel of four microsatellite loci, including SmBr18 and three previously published loci, has been used to characterise the nine Schistosoma species. Each species has been identified and classified based on its CA88 DNA fingerprint profile. Furthermore, microsatellite sequences and intra-specific variation have also been observed within the nine Schistosoma species sequences. Taken together, these results support the use of these markers in studying the population dynamics of Schistosoma isolates from endemic areas and also provide new methods for investigating the relationships between different populations of parasites. In addition, these data also indicate that Schistosoma magrebowiei check for this species in other resources is not a sister taxon to Schistosoma mattheei check for this species in other resources , prompting a new designation to a basal clade.

genotypes - microsatellite - Schistosoma - species - population dynamics

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