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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 4, 2010, pp. 485-487
Bioline Code: oc10083
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 4, 2010, pp. 485-487

 en Preliminary studies investigating the occurrence of Biomphalaria cousini check for this species in other resources in Brazil
Caldeira, Roberta Lima; Teodoro, Tatiana Maria; Gomes, Maria Flávia Belfort & Carvalho, Omar dos Santos

Abstract

Specific genetic profiles of Brazilian Biomphalaria check for this species in other resources species were previously standardized by molecular taxonomy through the analysis of restriction fragments, which were generated by digesting the internal transcribed spacer re- gion of rDNA with the DdeI endonuclease. Biomphalaria amazonica check for this species in other resources displayed three distinct profiles. To investigate these distinct profiles, the same molecular technique, polymerase chain reaction and restriction fragment length polymorphism, was used with different endonucleases. In addition, morphological data were also used to compare B. amazonica specimens that were collected from Brazil, Colombia and Bolivia. The morphological characters of Bolivian molluscs were similar to B. amazonica, displayed a molecular profile of five restriction fragments and morphological data, whereas the Colombian mollusc population showed morphological characters similar to Biomphalaria cousini check for this species in other resources and a molecular profile of three restriction fragments, similar to B. cousini. The Brazilian specimens showed the B. amazonica and B. cousini molecular profiles as well as a third profile, which resembled a combination of the Colombian and Bolivian molecular profiles.

Keywords
Biomphalaria cousin - Biomphalaria amazonica - PCR-RFLP - morphological characters

 
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