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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 5, 2010, pp. 627-632
Bioline Code: oc10106
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 5, 2010, pp. 627-632

 en Mycobacterium leprae check for this species in other resources downregulates the expression of PHEX in Schwann cells and osteoblasts
Silva, Sandra R. Boiça; Tempone, Antônio J.; Silva, Tatiana P.; Costa, Maria Renata S.N.; Pereira, Geraldo M.B.; Lara, Flávio A.; Pessolani, Maria Cristina V. & Esquenazi, Danuza

Abstract

Neuropathy and bone deformities, lifelong sequelae of leprosy that persist after treatment, result in significant impairment to patients and compromise their social rehabilitation. Phosphate-regulating gene with homologies to endopeptidase on the X chromosome (PHEX) is a Zn-metalloendopeptidase, which is abundantly expressed in osteoblasts and many other cell types, such as Schwann cells, and has been implicated in phosphate metabolism and X-linked rickets. Here, we demonstrate that Mycobacterium leprae check for this species in other resources stimulation downregulates PHEX transcription and protein expression in a human schwannoma cell line (ST88-14) and human osteoblast lineage. Modulation of PHEX expression was observed to a lesser extent in cells stimulated with other species of mycobacteria, but was not observed in cultures treated with latex beads or with the facultative intracellular bacterium Salmonella typhimurium check for this species in other resources . Direct downregulation of PHEX by M. leprae could be involved in the bone resorption observed in leprosy patients. This is the first report to describe PHEX modulation by an infectious agent.

Keywords
Mycobacterium leprae - leprosy - PHEX - osteoblasts - Schwann cells

 
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