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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 5, 2010, pp. 657-660
Bioline Code: oc10110
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 5, 2010, pp. 657-660

 en Helicobacter pylori check for this species in other resources transiently in the mouth may participate in the transmission of infection
Silva, Denise G.; Tinoco, Eduardo M.B.; Rocha, Gifone A.; Rocha, Andreia Maria Camargos; Guerra, Juliana B.; Saraiva, Ivan E.B. & Queiroz, Dulciene M.M.

Abstract

Helicobacter pylori check for this species in other resources infection is associated with peptic ulcer and gastric carcinoma. The oral cavity may be a reservoir for H. pylori; however, the results of studies on this subject are controversial. We employed single-step and nested polymerase chain reactions (PCR) to detect the presence of the vacA, ureA and 16S rDNA genes of H. pylori in the stomach, saliva and dental plaque of 30 subjects. The results were confirmed by sequencing. Nested 16S rDNA and ureA amplification was achieved in 80% of gastric, 30% of saliva and 20% of dental plaque specimens. Sequencing of 10, seven and four 16S rDNA products from stomach, saliva and dental plaque, respectively, showed > 99% identity with H. pylori. Sequencing of the other four oral cavity PCR products showed similarity with Campylobacter check for this species in other resources and Wolinella check for this species in other resources species. Additionally, the vacA genotype identified in the samples of different sites was the same within a given subject.H. pylori may be found in the oral cavity of patients with gastric infection, thus it could be a source of transmission. However, results obtained with detection methods based only on PCR should be interpreted with caution because other microorganisms that are phylogenetically very close to H. pylori are also present in the mouth.

Keywords
H. pylori - dental plaque - saliva - oral cavity - transmission - vacA

 
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