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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 5, 2010, pp. 687-691
Bioline Code: oc10116
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 5, 2010, pp. 687-691

 en Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi check for this species in other resources infection
Eickhoff, Christopher S.; Giddings, Olivia K.; Yoshida, Nobuko & Hoft, Daniel F.

Abstract

The potential use of the Trypanosoma cruzi check for this species in other resources metacyclic trypomastigote (MT) stage-specific molecule glycoprotein-82 (gp82) as a vaccine target has not been fully explored. We show that the opsonization of T. cruzi MT with gp82-specific antibody prior to mucosal challenge significantly reduces parasite infectivity. In addition, we investigated the immune responses as well as the systemic and mucosal protective immunity induced by intranasal CpG-adjuvanted gp82 vaccination. Spleen cells from mice immunized with CpG-gp82 proliferated and secreted IFN-γ in a dose-dependent manner in response to in vitro stimulation with gp82 and parasite lysate. More importantly, these CpG-gp82-immunized mice were significantly protected from a biologically relevant oral parasite challenge.

Keywords
Trypanosoma cruzi - mucosal immunity - vaccines

 
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