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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 6, 2010, pp. 736-745
Bioline Code: oc10125
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 6, 2010, pp. 736-745

 en Low and high-dose intradermal infection with Leishmania major check for this species in other resources and Leishmania amazonensis check for this species in other resources in C57BL/6 mice
Côrtes, Denise Fonseca; Carneiro, Matheus Batista Heitor; Santos, Liliane Martins; de Oliveira Souza, Talita Correia; Maioli, Tatiane Uceli; Duz, Ana Luiza C.; Ramos-Jorge, Maria Letícia; Afonso, Luis Carlos Crocco; Carneiro, Claudia & Vieira, Leda Quercia

Abstract

A model of skin infection with Leishmania amazonensis check for this species in other resources with low doses of parasites is compared to infection with high doses of L. amazonensis and low and high doses of Leishmania major check for this species in other resources . C57BL/6 mice were infected with 103 or 106 parasites in the ear and the outcome of infection was assessed. The appearance of lesions in mice infected with 103 parasites was delayed compared to mice infected with 106 Leishmania and parasites were detectable at the infection site before lesions became apparent. Mice infected with L. amazonensis displayed persistent lesions, whereas infection with L. major spontaneously healed in all groups, although lymphocytes persisted at the site of infection after healing. Macrophages persisted only in L. amazonensis-infected mice. High-dose L. amazonensis-infected mice produced lower levels of IFN-γ and TNF than mice infected with L. major. No correlation between the persistence of parasites and IL-10 levels and the production of nitric oxide or urea by macrophages was found. We conclude that infection with low doses of L. amazonensisin the dermis changes the course of infection by delaying the appearance of lesions. However, low-dose infection does not change the outcomes of susceptibility and cytokine production described for subcutaneous infection with high numbers of parasites.

Keywords
Leishmania - L. amazonensis - L. major - infection - cytokines

 
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