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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 6, 2010, pp. 823-828
Bioline Code: oc10139
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 6, 2010, pp. 823-828

 en Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus check for this species in other resources , Mansonella ozzardi check for this species in other resources and Mansonella perstans check for this species in other resources in the Amazon Region
Tang, Thuy-Huong Ta; López-Vélez, Rogelio; Lanza, Marta; Shelley, Anthony John; Rubio, Jose Miguel & Luz, Sérgio Luiz Bessa

Abstract

We present filaria-nested polymerase chain reaction (PCR), which is based on amplification of first internal transcribed spacer rDNA to distinguish three parasitic filarial species ( Onchocerca volvulus check for this species in other resources , Mansonella ozzardi check for this species in other resources and Mansonella perstans check for this species in other resources ) that can be found in the Amazon Region. Nested PCR-based identifications yielded the same results as those utilizing morphological characters. Nested PCR is highly sensitive and specific and it detects low-level infections in both humans and vectors. No cross-amplifications were observed with various other blood parasites and no false-positive results were obtained with the nested PCR. The method works efficiently with whole-blood, blood-spot and skin biopsy samples. Our method may thus be suitable for assessing the efficacy of filaria control programmes in Amazonia by recording parasite infections in both the human host and the vector. By specifically differentiating the major sympatric species of filaria, this technique could also enhance epidemiological research in the region.

Keywords
nested PCR - 18S rDNA - ITS-1 - onchocerciasis - mansonelliasis - Brazil

 
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