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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 7, 2010, pp. 899-903
Bioline Code: oc10152
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 7, 2010, pp. 899-903

 en Molecular comparison of topotypic specimens confirms Anopheles ( Nyssorhynchus) dunhami check for this species in other resources Causey (Diptera: Culicidae) in the Colombian Amazon
Ruiz, Freddy; Linton, Yvonne-Marie; Ponsonby, David J.; Conn, Jan E.; Herrera, Manuela; Quiñones, Martha L.; Vélez, Iván D & Wilkerson, Richard C

Abstract

The presence of Anopheles ( Nyssorhynchus) dunhami check for this species in other resources Causey in Colombia (Department of Amazonas) is confirmed for the first time through direct comparison of mtDNA cytochrome c oxidase I (COI) barcodes and nuclear rDNA second internal transcribed spacer (ITS2) sequences with topotypic specimens of An. dunhami from Tefé, Brazil. An. dunhami was identified through retrospective correlation of DNA sequences following misidentification as Anopheles nuneztovari s.l. using available morphological keys for Colombian mosquitoes. That An. dunhami occurs in Colombia and also possibly throughout the Amazon Basin, is of importance to vector control programs, as this non-vector species is morphologically similar to known malaria vectors including An. nuneztovari, Anopheles oswaldoi and Anopheles trinkae. Species identification of An. dunhami and differentiation from these closely related species are highly robust using either DNA ITS2 sequences or COI DNA barcode. DNA methods are advocated for future differentiation of these often sympatric taxa in South America.

Keywords
Anopheles dunhami - COI barcodes - ITS2 - topotypic - Brazil - Colombia

 
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