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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 7, 2010, pp. 935-937
Bioline Code: oc10160
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 7, 2010, pp. 935-937

 en Real-time PCR-based quantification of Toxoplasma gondii check for this species in other resources in tissue samples of serologically positive outdoor chickens
Aigner, Cleiton Paulo; da Silva, Aristeu Vieira; Sandrini, Fabiano; de Sá Osório, Paulo; Poiares, Lilian & Largura, Alvaro

Abstract

This study aimed to quantify Toxoplasma gondii check for this species in other resources in tissue samples of serologically positive chickens using real-time polymerase chain reaction (PCR). Of 65 chickens evaluated, 28 were positive for T. gondii antibodies. Brain and heart samples were collected from 26 seropositive chickens and DNA was extracted using Trizol® and amplified using real-time PCR with SYBR® Green. Parasite DNA was detected in 24 of the 26 samples analyzed; the number of positive tissue samples and the parasite quantity did not differ between tissue types. The results confirmed the analytical sensitivity of parasite detection in chicken tissue samples and demonstrated the possibility of using other molecular systems for genotypic analysis

Keywords
Toxoplasma gondii - chicken - real-time PCR

 
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