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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 8, 2010, pp. 1001-1009
Bioline Code: oc10172
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 8, 2010, pp. 1001-1009

 en Confirmation of Anopheles (Anopheles) calderoni check for this species in other resources Wilkerson, 1991 (Diptera: Culicidae) in Colombia and Ecuador through molecular and morphological correlation with topotypic material
González, Ranulfo; Carrejo, Nancy; Wilkerson, Richard C.; Alarcon, Joubert; Alarcon-Ormasa, Joubert; Ruiz, Fredy; Bhatia, Ravinder; Loaiza, José & Linton, Yvonne-Marie

Abstract

The morphologically similar taxa Anopheles calderoni check for this species in other resources , Anopheles punctimacula check for this species in other resources , Anopheles malefactor check for this species in other resources and Anopheles guarao check for this species in other resources are commonly misidentified. Isofamilies collected in Valle de Cauca, Colombia, showed morphological characters most similar to An. calderoni, a species which has never previously been reported in Colombia. Although discontinuity of the postsubcostal pale spots on the costa (C) and first radial (R1 ) wing veins is purportedly diagnostic for An. calderoni, the degree of overlap of the distal postsubcostal spot on C and R1 were variable in Colombian specimens (0.003-0.024). In addition, in 98.2% of larvae, seta 1-X was located off the saddle and seta 3-C had 4-7 branches in 86.7% of specimens examined. Correlation of DNA sequences of the second internal transcribed spacer and mtDNA cytochrome c oxidase subunit I gene (COI) barcodes (658 bp of the COI gene) generated from Colombian progeny material and wild-caught mosquitoes from Ecuador with those from the Peruvian type series of An. calderoni confirmed new country records. DNA barcodes generated for the closely related taxa, An. malefactor and An. punctimacula are also presented for the first time. Examination of museum specimens at the University of the Valle, Colombia, revealed the presence of An. calderoni in inland localities across Colombia and at elevations up to 1113 m.

Keywords
Anopheles calderoni - morphology - DNA barcodes - COI - ITS2 - Colombia - Ecuador - new country records

 
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