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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 8, 2010, pp. 1026-1032
Bioline Code: oc10175
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 8, 2010, pp. 1026-1032

 en In vitro effects of citral on Trypanosoma cruzi check for this species in other resources metacyclogenesis
Cardoso, Josiane & Soares, Maurilio José


Citral, the main constituent of lemongrass ( Cymbopogon citratus check for this species in other resources ) essential oil, was added to Trypanosoma cruzi check for this species in other resources cultures grown in TAU3AAG medium to observe the effect on the epimastigote-to-trypomastigote differentiation pro- cess (metacyclogenesis). Our results showed that citral (20 μg/mL) did not affect epimastigote viability or inhibit the differentiation process. Concentrations higher than 60 μg/mL, however, led to 100% cell death (both epimastigote and trypomastigote forms). Although epimastigotes incubated with 30 μg/mL citral were viable and able to adhere to the substrate, we observed around 50% inhibition in metacyclogenesis, with a calculated concentration that inhibited metacyclogenesis by 50% after 24 h (IC50 /24 h) of about 31 µg/mL. Treatment with 30 μg/mL citral did not hinder epimastigote multiplication because epimastigote growth resumed when treated cells were transferred to a drug- free liver infusion tryptose culture medium. Metacyclogenesis was almost totally abolished at 40 μg/mL after 24 h of incubation. Furthermore, the metacyclic trypomastigotes obtained in vitro were similarly susceptible to citral, with an IC50 /24 h, concentration that killed 50% of the cells after 24 h, of about 24.5 µg/mL. Therefore, citral appears to be a good candidate as an inhibitory drug for further studies analyzing the T. cruzi metacyclogenesis process.

citral - essential oil - metacyclogenesis - Trypanosoma cruzi

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