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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 8, 2010, pp. 1033-1039
Bioline Code: oc10176
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 8, 2010, pp. 1033-1039

 en The molecular detection of different Leishmania check for this species in other resources species within sand flies from a cutaneous and visceral leishmaniasis sympatric area in Southeastern Brazil
Saraiva, Lara; Filho, José Dilermando Andrade; Silva, Soraia de Oliveira; de Andrade, Antero Silva Ribeiro & Melo, Maria Norma

Abstract

Over the last 20 years, there has been an increase in the number of leishmaniasis cases in Brazil. Belo Horizonte (BH) is one of the most highly populated Brazilian cities that is affected by visceral leishmaniasis (VL). The health services in BH are coordinated by a central nucleus that is subdivided into nine sanitary districts. Historically, the highest level of human VL cases was found in the northeast sanitary district (NSD). The objective of our study was to detect Leishmania check for this species in other resources infection in the phlebotomine sand flies collected in the NSD by dissection and molecular ap- proaches. Following the occurrence of human VL cases in 2005, entomological captures were performed from July 2006-June 2007. Out of the 245 sand flies dissected, only three Lutzomyia longipalpis check for this species in other resources spp contained f lagellates. The female sand flies were grouped into 120 pools according to date, collection site and species, with approximately 10 individual sand flies in each pool. Subsquently, the DNA was extracted and Leishmania spp and other parasites were detected and identified by polymerase chain reaction (PCR) and PCR-restriction fragment length polymorfism. Leishmania infantum check for this species in other resources was present in at least 19% of the Lu. longipalpis collected, in 3.8% of the Nyssomiya whitmani check for this species in other resources collected, in 33.3% of the Evandromiya termitophila check for this species in other resources collected and in 14.3% of the Nyssomiya intermedia check for this species in other resources collected. When the females of the cortelezzii complex were compared with each other, 3.2% of the females were infected with Leishmania braziliensis check for this species in other resources , whereas 3.2% of the females were infected with trypanosomatids.

Keywords
Leishmania spp - sand flies - natural infection - Belo Horizonte

 
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