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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 106, No. 2, 2011, pp. 119-122
Bioline Code: oc11020
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 2, 2011, pp. 119-122

 en Antimicrobial resistance profiles and genetic characterisation of macrolide resistant isolates of Streptococcus agalactiae
Nakamura, Priscila A.M.; Schuab, Rôde Beatriz B.; Neves, Felipe P.G.; Pereira, Cláudio F.A.; de Paula, Geraldo R. & Barros, Rosana R

Abstract

In this study, 100 clinical isolates of Streptococcus agalactiae recovered from genitourinary tract specimens of non-pregnant individuals living in Rio de Janeiro were submitted for antimicrobial susceptibility testing, detection of macrolide resistance genes and evaluation of the genetic diversity of erythromycin-resistant isolates. By agar diffusion method, all isolates were susceptible to ceftazidime, penicillin and vancomycin. Isolates were resistant to levofloxacin (1%), clindamycin (5%), erythromycin (11%) and tetracycline (83%) and were intermediated to erythromycin (4%) and tetracycline (6%). Erythromycin-resistant and intermediated isolates presented the following phenotypes: M (n = 3), constitutive macrolide-lincosamide-streptogramin B (MLSB, n = 5) and inductive MLSB (n = 7). Determinants of macrolide resistance genes, erm and mef, were detected in isolates presenting MLSB and M phenotypes, respectively. Randomly amplified polymorphic DNA profiles of erythromycin-resistant isolates were clustered into two major groups of similarity.

Keywords
Streptococcus agalactiae - antimicrobial resistance - erythromycin resistance genes

 
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