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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 106, No. 2, 2011, pp. 182-189
Bioline Code: oc11030
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 2, 2011, pp. 182-189

 en An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens
Teixeira, Márcia Cristina Aquino; de Sá Oliveira, Geraldo Gileno; Santos, Patrícia Oliveira Meira; Bahiense, Thiago Campanharo; da Silva, Virginia Maria Goes; Rodrigues, Márcio Silva; Larangeira, Daniela Farias; dos-Santos, Washington Luis Conrado & Pontes-de-Carvalho, Lain Carlos

Abstract

Domestic dogs are considered to be the main reservoirs of zoonotic visceral leishmaniasis. In this work, we evaluated a protocol to induce Leishmania infantum check for this species in other resources / Leishmania chagasi check for this species in other resources -specific cellular and humoral immune responses in dogs, which consisted of two injections of Leishmania promastigote lysate followed by a subcutaneous inoculation of viable promastigotes. The primary objective was to establish a canine experimental model to provide positive controls for testing immune responses to Leishmania in laboratory conditions. After inoculation of viable promastigotes, specific proliferative responses of peripheral blood mononuclear cells (PBMCs) to either Leishmania lysate or recombinant proteins, the in vitro production of interferon-γ by antigen-stimulated PBMCs and a significant increase in circulating levels of anti-Leishmania antibodies were observed. The immunized dogs also displayed positive delayed-type hypersensitivity reactions to Leishmania crude antigens and to purified recombinant proteins. An important finding that supports the suitability of the dogs as positive controls is that they remained healthy for the entire observation period, i.e., more than seven years after infection. Following the Leishmania antigen lysate injections, the infection of dogs by the subcutaneous route appears to induce a sustained cellular immune response, leading to an asymptomatic infection. This provides a useful model for both the selection of immunogenic Leishmania antigens and for immunobiological studies on their possible immunoprotective activities.

Keywords
Leishmania infantum - Leishmania chagasi - dogs - immunization - cellular immune response

 
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