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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 106, No. 2, 2011, pp. 190-193
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Bioline Code: oc11031
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 2, 2011, pp. 190-193
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Correlation of meta 1 expression with culture stage, cell morphology and infectivity in Leishmania (Leishmania) amazonensis promastigotes
dos Santos, Marcos Gonzaga; da Silva, Maria Fernanda Laranjeira; Zampieri, Ricardo Andrade; Lafraia, Rafaella Marino & Floeter-Winter, Lucile Maria
Abstract
The parasitic protozoan Leishmania (Leishmania) amazonensis alternates between mammalian and insect hosts. In the insect host, the parasites proliferate as procyclic promastigotes andthen differentiate into metacyclic infective forms. The meta 1 gene is preferentially expressed during metacyclogenesis. meta 1 expression profile determination along parasite growth curves revealed that the meta 1 mRNA level peaked at the early stationary phase then decreased to an intermediate level. No correlation was observed between meta 1 expression and infectivity. Conversely, infectivity correlated with the increase of apoptotic cells in the late stationary phase.
Keywords
meta 1 expression - metacyclic morphology - phosphatidylserine exposure
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