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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 106, No. 2, 2011, pp. 200-206
Bioline Code: oc11033
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 2, 2011, pp. 200-206

 en Identification of candidate antigens from adult stages of Toxocara canis check for this species in other resources for the serodiagnosis of human toxocariasis
Peixoto, Patrícia Longuinhos; Nascimento, Evaldo; Cançado, Guilherme Grossi Lopes; de Miranda, Rodrigo Rodrigues Cambraia; Rocha, Regina Lunardi; Araújo, Ricardo Nascimento & Fujiwara, Ricardo Toshio

Abstract

In the present work, we identified adult Toxocara canis check for this species in other resources antigens through sodium dodecyl sulfate-polyacrylamide gel electrophoresis for potential use in human toxocariasis immunodiagnosis. The sensitivity and specificity of several semi-purified antigens, as well as their cross-reactivity with other parasitic infections, were assessed by IgM and IgG-enzime linked immunosorbent assay. Whilst we found that the crude extract of the parasite presented limited sensitivity, specificity and high cross-reactivity against other parasites, we identified 42, 58, 68 and 97-kDa semi-purified antigens as the most promising candidates for immunodiagnosis. Moreover, the 58 and 68-kDa antigens presented the lowest IgM cross-reactivity. When tested as a combination, a mixture of the 58 and 68-kDa antigens presented 100% sensitivity and specificity, as well as minor cross-reactivity. Although the combination of the 42, 58, 68 and 97-kDa antigens presented 100% sensitivity at a dilution of 1:40, the low specificity and high cross-reactivity observed suggested a limited use for diagnostic purposes. Our data suggested that the 58 and 68-kDa antigens might be most suitable for the immunodiagnosis of human toxocariasis.

Keywords
Toxocara - ELISA - visceral larva migrans

 
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