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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 106, No. 2, 2011, pp. 248-250
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Bioline Code: oc11040
Full paper language: English
Document type: Short Communication
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 2, 2011, pp. 248-250
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2D-immunoblotting analysis of Sporothrix schenckii cell wall
Ruiz-Baca, Estela; Mora-Montes, Héctor M; Mojica-Marín, Virgilio & Urtiz-Estrada, Norma
Abstract
We utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting) with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell wall of both morphologies and that a 60-kDa glycoprotein was present only in yeast-like cells. In addition to the Gp70, the wall from filament cells showed four proteins with molecular weights of 48, 55, 66 and 67 kDa, some of which exhibited several isoforms. To our knowledge, this is the first 2D-immunoblotting analysis of the S. schenckii cell wall.
Keywords
sporotrichosis - Sporothrix schenckii - cell wall - glycoproteins - 2D-immunoblotting analysis
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