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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 106, No. 2, 2011, pp. 251-253
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Bioline Code: oc11041
Full paper language: English
Document type: Short Communication
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 2, 2011, pp. 251-253
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In vitro activity of amphotericin B cochleates against Leishmania chagasi
Sesana, Aretha Molina; Monti-Rocha, Renata; Vinhas, Solange Alves; Morais, Carlos Gustavo; Dietze, Reynaldo & Lemos, Elenice Moreira
Abstract
Cochleate delivery vehicles are a novel lipid-based system with potential for delivery of amphotericin B (AmB). In this study, the efficacy of cochleates was evaluated by examining the in vitro activity of AmB cochleates (CAMB) against Leishmania chagasi in a macrophage model of infection. We demonstrate that CAMB is nontoxic to macrophages at concentrations as high as 2.5 μg/mL, whereas the conventional formulation, AmB deoxycholate, showed high toxicity at this concentration. The in vitro activity of CAMB against L. chagasi was found to be similar to that of the reference drug AmB deoxycholate, with ED50s of 0.017 μg/mL and 0.021 μg/mL, respectively. Considering that L. chagasi affects organs amenable to cochleate-mediated delivery of AmB, we hypothesize that CAMB will be an effective lipid system for the treatment of visceral leishmaniasis.
Keywords
amphotericin B cochleates - Leishmania chagasi - in vitro activity
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