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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 106, No. 4, 2011, pp. 385-389
Bioline Code: oc11067
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 4, 2011, pp. 385-389

 en Evaluation of RbCl and CrCl3 as markers of Triatoma brasiliensis check for this species in other resources (Hemiptera: Reduviidae) nymphs: persistence and influence of Rb and Cr on triatomine biology
Sarquis, Otília; Oliveira, Lívia S.; Reginaldo, Rego; Gonçalves, Jaylei M.; Lima, Marli M. & Maciel-de-Freitas, Rafael

Abstract

In order to mark Triatoma brasiliensis check for this species in other resources , the vector of Chagas disease in Brazil, two chemical compounds, rubidium chloride (RbCl) and chromium chloride (CrCl3), were tested. First, 199 N2-N5 nymphs were fed on blood with 0.025M RbCl. Rb marker positivity ranged from 2.5% (N3)-70% (N2), with a maximum persistence of 98 days. Second, 265 N2-N5 nymphs were fed on blood containing 0.0015M CrCl3. Cr marker positivity ranged up to 93% (N5), with a maximum persistence of 119 days. Finally, we blood fed 213 T. brasiliensis to investigate whether CrCl3 altered the biology of this insect. The developmental time of T. brasiliensis was unaltered, but the survival of the Cr-marked group was lower than that of the control group. Differences in the mean fecundity of the control (mean of 156.1) and experimental (mean of 135.6) groups were not statistically significant and 100% of the egg batches of females Cr-marked as nymphs were positive. In conclusion, CrCl3 is a useful tool for marking T. brasiliensis nymphs due to its high positivity and persistence.

Keywords
Triatoma brasiliensis, marking, survival, fecundity, development

 
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