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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 106, No. 4, 2011, pp. 394-399
Bioline Code: oc11069
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 4, 2011, pp. 394-399

 en An MLSA-based online scheme for the rapid identification of Stenotrophomonas isolates
Ramos, Patrícia Locosque; Moreira-Filho, Carlos Alberto; Van Trappen, Stefanie; Swings, Jean; De Vos, Paul; Barbosa, Heloiza Ramos; Thompson, Cristiane Carneiro; Vasconcelos, Ana Tereza Ribeiro & Thompson, Fabiano Lopes

Abstract

An online scheme to assign Stenotrophomonas isolates to genomic groups was developed using the multilocus sequence analysis (MLSA), which is based on the DNA sequencing of selected fragments of the housekeeping genes ATP synthase alpha subunit (atpA), the recombination repair protein (recA), the RNA polymerase alpha subunit (rpoA) and the excision repair beta subunit (uvrB). This MLSA-based scheme was validated using eight of the 10 Stenotrophomonas species that have been previously described. The environmental and nosocomial Stenotrophomonas strains were characterised using MLSA, 16S rRNA sequencing and DNA-DNA hybridisation (DDH) analyses. Strains of the same species were found to have greater than 95% concatenated sequence similarity and specific strains formed cohesive readily recognisable phylogenetic groups. Therefore, MLSA appeared to be an effective alternative methodology to amplified fragment length polymorphism fingerprint and DDH techniques. Strains of Stenotrophomonas can be readily assigned through the open database resource that was developed in the current study (www.steno.lncc.br/).

Keywords
Stenotrophomonas, MLSA, online scheme

 
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