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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 106, No. 4, 2011, pp. 424-432
Bioline Code: oc11073
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 4, 2011, pp. 424-432

 en Interaction between primary and secondary sporocysts of Schistosoma mansoni check for this species in other resources and the internal defence system of Biomphalaria check for this species in other resources resistant and susceptible to the parasite
Alves de Mattos, Ana Carolina; Martins-Souza, Raquel Lopes; Kusel, John Robert & Coelho, Paulo Marcos Zech

Abstract

The outcome of the interaction between Biomphalaria and Schistosoma mansoni check for this species in other resources depends on the response of the host internal defence system (IDS) and the escape mechanisms of the parasite. The aim of this study was to evaluate the responsiveness of the IDS (haemocytes and soluble haemolymph factors) of resistant and susceptible Biomphalaria tenagophila check for this species in other resources lineages and Biomphalaria glabrata check for this species in other resources lineages in the presence of in vitro-transformed primary sporocysts and secondary sporocysts obtained from infected B. glabrata. To do this, we assayed the cellular adhesion index (CAI), analysed viability/mortality, used fluorescent markers to evaluate the tegumental damage and transplanted secondary sporocysts. B. tenagophila Taim was more effective against primary and secondary sporocystes than the susceptible lineage and B. glabrata. Compared with secondary sporocysts exposed to B. tenagophila, primary sporocysts showed a higher CAI, a greater percentage of dead sporocysts and were labelled by lectin from Glycine max and Alexa-Fluor 488 fluorescent probes at a higher rate than the secondary sporocysts. However, the two B. tenagophila lineages showed no cercarial shedding after inoculation with secondary sporocysts. Our hypothesis that secondary sporocysts can escape the B. tenagophila IDS cannot be confirmed by the transplantation experiments. These data suggest that there are additional mechanisms involved in the lower susceptibilty of B. tenagophila to S. mansoni infection.

Keywords
Schistosoma mansoni, sporocysts, Biomphalaria tenagophila, internal defence system, escape mechanisms

 
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