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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 106, No. 4, 2011, pp. 502-504
Bioline Code: oc11085
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 4, 2011, pp. 502-504

 en Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
de Mendonça, Marcos César Lima; de Amorim Ferreira, Ana Maria; dos Santos, Marta Gonçalves Matos; Oviedo,Elva Cristina; Dal Bello, Maria Sônia; Siqueira, Marilda Mendonça; Maceira, Juan Manuel Piñeiro; von Hubinger, Maria Genoveva & Couceiro,José Nelson dos Santos Silva

Abstract

Heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1.

Keywords
parvovirus B19, nucleotide sequencing, genotyping, HMA, SSCP

 
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