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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 106, No. 6, 2011, pp. 748-754
Bioline Code: oc11126
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 6, 2011, pp. 748-754

 en The presence of Helicobacter pylori check for this species in other resources in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient
Silva, Luciana; Rocha, Andreia; Rocha, Gifone; Moura, Sílvia; Rocha, Márcia; Melo, Fabrício; Guerra, Juliana; Castro, Lúcia; Mendes, Guilherme; Ferrari, Teresa; Lima, Agnaldo & Queiroz, Dulciene

Abstract

The hypothesis that Helicobacter check for this species in other resources might be a risk factor for human liver diseases has arisen after the detection of Helicobacter DNA in hepatic tissue of patients with hepatobiliary diseases. Nevertheless, no explanation that justifies the presence of the bacterium in the human liver has been proposed. We evaluated the presence of Helicobacter in the liver of patients with hepatic diseases of different aetiologies. We prospectively evaluated 147 patients (106 with primary hepatic diseases and 41 with hepatic metastatic tumours) and 20 liver donors as controls. Helicobacter species were investigated in the liver by culture and specific 16S rDNA nested-polymerase chain reaction followed by sequencing. Serum and hepatic levels of representative cytokines of T regulatory cell, T helper (Th)1 and Th17 cell lineages were determined using enzyme linked immunosorbent assay. The data were evaluated using logistic models. Detection of Helicobacter pylori check for this species in other resources DNA in the liver was independently associated with hepatitis B virus/hepatitis C virus, pancreatic carcinoma and a cytokine pattern characterised by high interleukin (IL)-10, low/absent interferon-γ and decreased IL-17A concentrations (p 10-3). The bacterial DNA was never detected in the liver of patients with alcoholic cirrhosis and autoimmune hepatitis that are associated with Th1/Th17 polarisation. H. pylori may be observed in the liver of patients with certain hepatic and pancreatic diseases, but this might depend on the patient cytokine profile.

Keywords
Helicobacter pylori, liver diseases, IL-10, IL-17, IFN-γ

 
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