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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 106, No. 6, 2011, pp. 763-768
Bioline Code: oc11129
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 6, 2011, pp. 763-768

 en Survey of Leptospira check for this species in other resources spp in pampas deer ( Ozotoceros bezoarticus check for this species in other resources ) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction
Vieira, Anahí; Rosinha, Grácia; Oliveir, Carina; Vasconcellos, Silvio; Lima-Borges, Paulo; Tomás, Walfrido; Mourão, Guilherme; Lacerda, Ana; Soares, Cleber; Araújo, Flábio; Piovezan, Ubiratan; Zucco, Carlos & Pellegrin, Aiesca

Abstract

This work reports a survey of Leptospira check for this species in other resources spp in pampas deer ( Ozotoceros bezoarticus check for this species in other resources ) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction (PCR). Seventy pampas deer were captured in the dry season and surveyed using PCR, microscopic agglutination test (MAT) (n = 51) and by both techniques (n = 47). PCR detected infections in two pampas deer and MAT detected infections in three. Through sequencing and phylogenetic analyses, the PCR-amplified fragment detected in deer was identified as Leptospira interrogans check for this species in other resources . Serovars Pomona and Butembo were detected using MAT and the highest titre was 200 for serovar Pomona. Epidemiological aspects of the findings are discussed.

Keywords
pampas deer, Leptospira spp, MAT, PCR, Pantanal

 
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