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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 106, No. s1, 2011, pp. 27-33
Bioline Code: oc11137
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 106, No. s1, 2011, pp. 27-33

 en Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax check for this species in other resources in Brazilian field isolates
Ribeiro, Ricardo Souza; Ladeira, Luisa; Rezende, Antonio Mauro; Fontes, Cor Jesus Fernandes; Carvalho, Luzia Helena & Brito, Cristiana Ferreira Alves de


Reliable molecular markers are essential for a better understanding of the molecular epidemiology of Plasmodium vivax check for this species in other resources , which is a neglected human malaria parasite. The aim of this study was to analyze the genetic diversity of P. vivax isolates from the Brazilian Amazon using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the highly polymorphic merozoite surface protein-3alpha (PvMSP-3α) gene. To accomplish this, 60 isolates of P. vivax from different endemic areas in the Brazilian Amazon were collected. The PvMSP-3α gene was amplified by nested-PCR. Three major types of the PvMSP-3α locus were detected at different frequencies: type A (68%), B (15%) and C (17%). A single sample showed two PCR fragments, which corresponded to infection with types A and C. PCR-RFLP analysis using the HhaI restriction enzyme for 52 isolates clearly identified 11 haplotypes, eight of which were from type A, two from type B and only one from type C. Seven other isolates did not show a clear pattern using PCR-RFLP. This result might be due to multiple clone infections. This study showed a high diversity of the PvMSP-3α gene among P. vivax isolates from the Brazilian Amazon, but also indicated that the detection performance of PCR-RFLP of the PvMSP-3α gene may not be sufficient to detect multiple clone infections.

malaria, Plasmodium vivax, genetic variation, antigenic variability, MSP3 protein, restriction fragment length polymorphism

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