Anopheles triannulatus
s.l. is a malaria vector with a wide geographic distribution, ranging from Argentina-Nicaragua and Trinidad. Here we analysed sequences of two genes, timeless and cpr, to assess the genetic variability and divergence among three sympatric cryptic species of this complex from Salobra, central-western Brazil. The timeless gene sequences did not conclusively differentiate
Anopheles halophylus
and
An. triannulatus species "C". However, a partial separation has been observed between these species and
An. triannulatus s.s. Importantly, the analysis of the cpr gene sequences revealed fixed differences, no shared polymorphisms and considerable genetic differentiation among the three species of the An. triannulatus complex. The results confirm that
An. triannulatus s.s.,
An. halophylus and
An. triannulatus species C are distinct taxa, with the latter two likely representing a more recent speciation event.
