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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 106, No. 7, 2011
Bioline Code: oc11177
Full paper language: English
Document type: Study
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 7, 2011

 en Participation of N-acetyl-D-glucosamine carbohydrate moieties in the recognition of Schistosoma mansoni check for this species in other resources sporocysts by haemocytes of Biomphalaria tenagophila check for this species in other resources
Martins-Souza, Raquel Lopes; Pereira, Cintia Aparecida Jesus; Rodrigues, Leonardo; Araújo, Emília Souza; Coelho, Paulo Marcos Zech; Corrêa Jr, Ary & Negrão-Corrêa, Deborah

Abstract

Lectin-carbohydrate binding may be involved in the recognition of Schistosoma mansoni check for this species in other resources sporocysts by haemocytes of Biomphalaria; therefore, we tested if this interaction is associated with snail resistance against Schistosoma infection. In vitro data showed that most of the S. mansoni sporocysts cultured with haemocytes from Biomphalaria glabrata check for this species in other resources BH, a highly susceptible snail strain, had a low number of cells that adhered to their tegument and a low mortality rate. Moreover, the addition of N-acetyl-D-glucosamine (GlcNAc) did not alter this pattern of adherence and mortality. Using haemocytes and haemolymph of Biomphalaria tenagophila check for this species in other resources Cabo Frio, we observed a high percentage of sporocysts with adherent cells, but complete encapsulation was not detected. Low concentrations of GlcNAc increased haemocyte binding to the sporocysts and mortality, which returned to basal levels with high concentrations of the carbohydrate. In contrast, haemocytes plus haemolymph from B. tenagophila Taim encapsulated cellular adhesion index of level 3 and destroyed over 30% of the S. mansoni sporocysts in culture. Interestingly, the addition of GlcNAc, but not mannose, to the culture medium resulted in the significant inhibition of cellular adhesion to the parasite tegument and the reduction of parasite mortality, suggesting that GlcNAc carbohydrate moieties are important to the recognition of S. mansoni by B. tenagophila Taim

Keywords
Schistosoma mansoni; Biomphalaria tenagophila; snail susceptibility to trematode; N-acetyl-D-glucosamine; host-parasite recognition

 
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