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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 106, No. 8, 2011, pp. 948-956
Bioline Code: oc11188
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 8, 2011, pp. 948-956

 en Biological, biochemical and molecular features of Trypanosoma cruzi check for this species in other resources strains isolated from patients infected through oral transmission during a 2005 outbreak in the state of Santa Catarina, Brazil: its correspondence with the new T. cruzi Taxonomy Consensus (2009)
Andrade, Sonia Gumes; Campos, Rozália Figueira; Steindel, Mario; Guerreiro, Marcos Lázaro; Magalhães, Juracy Barbosa; de Almeida, Marcio Cerqueira; Reis, Joice Neves; Santos, Viviane Corrêa; Valadares, Helder Magno Silva; dos Reis, Mitermayer Galvão & Macedo, Andréa Mara

Abstract

We examined strains of Trypanosoma cruzi check for this species in other resources isolated from patients with acute Chagas disease that had been acquired by oral transmission in the state of Santa Catarina, Brazil (2005) and two isolates that had been obtained from a marsupial ( Didelphis aurita check for this species in other resources ) and a vector ( Triatoma tibiamaculata check for this species in other resources ). These strains were characterised through their biological behaviour and isoenzymic profiles and genotyped according to the new Taxonomy Consensus (2009) based on the discrete typing unities, that is, T. cruzi genotypes I-VI. All strains exhibited the biological behaviour of biodeme type II. In six isolates, late peaks of parasitaemia, beyond the 20th day, suggested a double infection with biodemes II + III. Isoenzymes revealed Z2 or mixed Z1 and Z2 profiles. Genotyping was performed using three polymorphic genes (cytochrome oxidase II, spliced leader intergenic region and 24Sα rRNA) and the restriction fragment length polymorphism of the kDNA minicircles. Based on these markers, all but four isolates were characterised as T. cruzi II genotypes. Four mixed populations were identified: SC90, SC93 and SC97 (T. cruzi I + T. cruzi II) and SC95 (T. cruzi I + T. cruzi VI). Comparison of the results obtained by different methods was essential for the correct identification of the mixed populations and major lineages involved indicating that characterisation by different methods can provide new insights into the relationship between phenotypic and genotypic aspects of parasite behaviour.

Keywords
Trypanosoma cruzi strains - oral transmission - biodemes - genotyping - Taxonomy Consensus 2009

 
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