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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 106, No. 8, 2011, pp. 957-967
Bioline Code: oc11189
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 8, 2011, pp. 957-967

 en Search for a platelet-activating factor receptor in the Trypanosoma cruzi check for this species in other resources proteome: a potential target for Chagas disease chemotherapy
Kawano, Daniel Fábio; da Silva, Vinicius Barreto; de Melo Jorge, Daniel Macedo; de Paula da Silva, Carlos Henrique Tomich & Carvalho, Ivone

Abstract

Chagas disease(CD) causes the highest burden of parasitic diseases in the Western Hemisphere and is therefore a priority for drug research and development. Platelet-activating factor (PAF) causes the CD parasite Trypanosoma cruzi check for this species in other resources to differentiate, which suggests that the parasite may express PAF receptors. Here, we explored the T. cruzi proteome for PAF receptor-like proteins. From a total of 23,000 protein sequences, we identified 29 hypothetical proteins that are predicted to have seven transmembrane domains (TMDs), which is the main characteristic of the G protein-coupled receptors (GPCRs), including the PAF receptor. The TMDs of these sequences were independently aligned with domains from 25 animal PAF receptors and the sequences were analysed for conserved residues. The conservation score mean values for the TMDs of the hypothetical proteins ranged from 31.7-44.1%, which suggests that the putative T. cruzi PAF receptor is among the sequences identified, the TMDs are not highly conserved. These results suggest that T. cruzi contains several GPCR-like proteins and that one of these GPCRs may be a PAF receptor. Future studies may further validate the PAF receptor as a target for CD chemotherapy.

Keywords
PAF - GPCR - Trypanosoma cruzi - Chagas disease - bioinformatics - proteomeprocesses

 
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