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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 106, No. 8, 2011, pp. 1002-1006
Bioline Code: oc11196
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 8, 2011, pp. 1002-1006

 en The genetic diversity and phenotypic characterisation of Streptococcus agalactiae check for this species in other resources isolates from Rio de Janeiro, Brazil
Corrêa, Ana Beatriz de Almeida; da Silva, Lígia Guedes; Pinto, Tatiana de Castro Abreu; de Oliveira, Ivi Cristina Menezes; Fernandes, Flávio Gimenis; da Costa, Natalia Silva; de Mattos, Marcos Corrêa; Fracalanzza, Eduardo Longo & Benchetrit, Leslie Claude

Abstract

Streptococcus agalactiae check for this species in other resources isolates are more common among pregnant women, neonates and nonpregnant adults with underlying diseases compared to other demographic groups. In this study, we evaluate the genetic and phenotypic diversity in S. agalactiae strains from Rio de Janeiro (RJ) that were isolated from asymptomatic carriers. We analysed these S. agalactiae strains using pulsed-field gel electrophoresis(PFGE), serotyping and antimicrobial susceptibility testing, as well as by determining the macrolide resistance phenotype, and detecting the presence of the ermA/B, mefA/E and lnuB genes. The serotypes Ia, II, III and V were the most prevalent serotypes observed. The 60 strains analysed were susceptible to penicillin, vancomycin and levofloxacin. Resistance to clindamycin, chloramphenicol, erythromycin, rifampin and tetracycline was observed. Among the erythromycin and/or clindamycin resistant strains, the ermA, ermB and mefA/E genes were detected and the constitutive macrolides, lincosamides and streptogramin B-type resistance was the most prevalent phenotype observed. The lnuB gene was not detected in any of the strains studied. We found 56 PFGE electrophoretic profiles and only 22 of them were allocated in polymorphism patterns. This work presents data on the genetic diversity and prevalent capsular serotypes among RJ isolates. Approximately 85% of these strains came from pregnant women; therefore, these data may be helpful in developing future prophylaxis and treatment strategies for neonatal syndromes in RJ.

Keywords
Streptococcus agalactiae - pulsed-field gel electrophoresis - polymerase chain reaction - antimicrobial susceptibility

 
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