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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 106, No. 8, 2011, pp. 1014-1016
Bioline Code: oc11198
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 106, No. 8, 2011, pp. 1014-1016

 en Trypanosoma cruzi check for this species in other resources trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
Fernandes, Maria Cecília; de Andrade, Leonardo Rodrigues; Andrews, Norma Windsor & Mortara, Renato Arruda

Abstract

It has been recently shown that Trypanosoma cruzi check for this species in other resources trypomastigotes subvert a constitutive membrane repair mechanism to invade HeLa cells. Using a membrane extraction protocol and high-resolution microscopy, the HeLa cytoskeleton and T. cruzi parasites were imaged during the invasion process after 15 min and 45 min. Parasites were initially found under cells and were later observed in the cytoplasm. At later stages, parasite-driven protrusions with parallel filaments were observed, with trypomastigotes at their tips. We conclude that T. cruzi trypomastigotes induce deformations of the cortical actin cytoskeleton shortly after invasion, leading to the formation of pseudopod-like structures.

Keywords
Trypanosoma cruzi - cell invasion - cytoskeleton - membrane protrusion

 
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