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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 107, No. 1, 2012, pp. 39-47
Bioline Code: oc12006
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 107, No. 1, 2012, pp. 39-47

 en Genetic diversity of Leishmania infantum check for this species in other resources field populations from Brazil
Segatto, Marcela; Ribeiro, Lucas Secchim; Costa, Dorcas Lamounier; Costa, Carlos Henrique Nery; Oliveira, Márcia Rosa de; Carvalho, Sílvio Fernando Guimarães; Macedo, Andréa Mara; Valadares, Helder Magno Silva; Dietze, Reynaldo; Brito, Cristiana Ferreira Alves de & Lemos, Elenice Moreira

Abstract

Leishmania infantum check for this species in other resources (syn. Leishmania chagasi check for this species in other resources ) is the etiological agent of visceral leishmaniasis (VL) in Brazil. The epidemiology of VL is poorly understood. Therefore, a more detailed molecular characterization at an intraspecific level is certainly needed. Herein, three independent molecular methods, multilocus microsatellite typing(MLMT), random amplification of polymorphic DNA (RAPD) and simple sequence repeats-polymerase chain reaction(SSR-PCR), were used to evaluate the genetic diversity of 53 L. infantum isolates from five different endemic areas in Brazil. Population structures were inferred by distance-based and Bayesian-based approaches. Eighteen very similar genotypes were detected by MLMT, most of them differed in only one locus and no correlation was found between MLMT profiles, geographical origin or the estimated population structure. However, complex profiles composed of 182 bands obtained by both RAPD and SSR-PCR assays gave different results. Unweighted pair group method with arithmetic mean trees built from these data revealed a high degree of homogeneity within isolates of L. infantum. Interestingly, despite this genetic homogeneity, most of the isolates clustered according to their geographical origin.

Keywords
Leishmania infantum - genetic diversity - MLMT - RAPD - SSR-PCR

 
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