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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 107, No. 3, 2012, pp. 303-309
Bioline Code: oc12044
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 107, No. 3, 2012, pp. 303-309

 en Production of TNF-α, nitric oxide and hydrogen peroxide by macrophages from mice with paracoccidioidomycosis that were fed a linseed oil-enriched diet
Sargi, Sheisa Cyléia; Dalalio, Márcia Machado de Oliveira; Visentainer, Jesuí Vergílio; Bezerra, Rafael Campos; Perini, João Ângelo de Lima; Stevanato, Flávia Braidotti & Visentainer, Jeane Eliete Laguila

Abstract

Omega-3 polyunsaturated fatty acids (n-3 PUFA) can modulate the immune system and their primary effect is on macrophage function. Paracoccidioidomycosis (PCM) is an endemic systemic mycosis in Latin America that is caused by the dimorphic fungus Paracoccidioides brasiliensis check for this species in other resources (Pb). Macrophages are the main defence against this pathogen and have microbicidal activity that is dependent on interferon-γ and tumour necrosis factor (TNF)-α. These cytokines stimulate the synthesis of nitric oxide (NO) and hydrogen peroxide (H2O2), leading to the death of the fungus. To study the effect of n-3 PUFA on the host immune response during experimental PCM, macrophages that were obtained from animals infected with Pb18 and fed a diet enriched by linseed (LIN) oil were cultured and challenged with the fungus in vitro. The macrophage function was analysed based on the concentrations of TNF-α, NO and H2O2. LIN oil seems to influence the production of TNF-α during the development of disease. A diet enriched with LIN oil influences the microbicidal activity of the macrophages by inducing the production of cytokines and metabolites such as NO and H2O2, predominantly in the chronic phase of infection.

Keywords
Paracoccidioides brasiliensis - cytokines - n-3 polyunsaturated fatty acids

 
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