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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 107, No. 3, 2012, pp. 348-355
Bioline Code: oc12050
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 107, No. 3, 2012, pp. 348-355

 en Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus check for this species in other resources enterotoxin A in vitro during early and late episodes of chronic osteomyelitis
Ferreira, Gabriella Freitas; Moraes, Cícero; da Silveira, Alda Maria Soares; Correa-Oliveira, Rodrigo; Teixeira-Carvalho, Andréa; Martins-Filho, Olindo Assis; Moreno, Elizabeth Castro; do Carmo, Luiz Simeão; Fraga, Lucia Alves de Oliveira & Malaquias, Luiz Cosme Cotta

Abstract

We investigated the cytokine profile of peripheral mononuclear cells from chronic osteomyelitis (OST) patients following in vitro stimulation with staphylococcal enterotoxin A (SEA). We demonstrate that stimulation with SEA induced prominent lymphocyte proliferation and high levels of tumour necrosis factor (TNF)-α, interleukin (IL)-4 and IL-10 secretion in both OST and non-infected individuals (NI). Even though stimulation with SEA had no impact on IL-6 production in either patient group, the baseline level of IL-6 production by cells from OST patients was always significantly less than that produced by cells from NI. After classifying the osteomyelitic episodes based on the time after the last reactivation event as “early” (1-4 months) or “late” osteomyelitis (5-12 months), we found that increased levels of TNF-α and IL-4 in combination with decreased levels of IL-6 were observed in the early episodes. By contrast, increased levels of IL-10, IL-2 and IL-6 were hallmarks of late episodes. Our data demonstrate that early osteomyelitic episodes are accompanied by an increased frequency of “high producers” of TNF-α and IL-4, whereas late events are characterised by increased frequencies of “high producers” of IL-10, IL-6 and IL-2. These findings demonstrate the distinct cytokine profiles in chronic osteomyelitis, with a distinct regulation of IL-6 production during early and late episodes.

Keywords
Staphylococcus aureus - enterotoxin A - chronic osteomyelitis - cytokines - IL-6

 
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