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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 107, No. 4, 2012, pp. 486-493
Bioline Code: oc12071
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 107, No. 4, 2012, pp. 486-493

 en Aggregative adherent strains of Corynebacterium pseudodiphtheriticum check for this species in other resources enter and survive within HEp-2 epithelial cells
de Souza, Monica Cristina; dos Santos, Louisy Sanches; Gomes, Débora Leandro Rama; Sabbadini, Priscila Soares; dos Santos, Cinta Silva; Camello, Thereza Christina Ferreira; Asad, Lídia Maria Buarque Oliveira; Rosa, Ana Claúdia de Paula; Nagao, Prescilla Emy; Júnior, Raphael Hirata & Guaraldi, Ana Luiza de Mattos

Abstract

Corynebacterium pseudodiphtheriticum check for this species in other resources is a well-known human pathogen that mainly causes respiratory disease and is associated with high mortality in compromised hosts. Little is known about the virulence factors and pathogenesis of C. pseudodiphtheriticum. In this study, cultured human epithelial (HEp-2) cells were used to analyse the adherence pattern, internalisation and intracellular survival of the ATCC 10700 type strain and two additional clinical isolates. These microorganisms exhibited an aggregative adherence-like pattern to HEp-2 cells characterised by clumps of bacteria with a “stacked-brick” appearance. The differences in the ability of these microorganisms to invade and survive within HEp-2 cells and replicate in the extracellular environment up to 24 h post infection were evaluated. The fluorescent actin staining test demonstrated that actin polymerisation is involved in the internalisation of the C. pseudodiphtheriticum strains. The depolymerisation of microfilaments by cytochalasin E significantly reduced the internalisation of C. pseudodiphtheriticum by HEp-2 cells. Bacterial internalisation and cytoskeletal rearrangement seemed to be partially triggered by the activation of tyrosine kinase activity. Although C. pseudodiphtheriticum strains did not demonstrate an ability to replicate intracellularly, HEp-2 cells were unable to fully clear the pathogen within 24 h. These characteristics may explain how some C. pseudodiphtheriticum strains cause severe infection in human patients.

Keywords
aggregative-like adherence pattern - Corynebacterium pseudodiphtheriticum - HEp-2 cells - intracellular survival - persistence

 
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