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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 107, No. 4, 2012, pp. 510-512
Bioline Code: oc12074
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 107, No. 4, 2012, pp. 510-512

 en Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis
Silveira-Gomes, Fabíola & Marques-da-Silva, Silvia Helena

Abstract

Paracoccidioidomycosis is diagnosed from the direct observation of the causative agent, but serology can facilitate and decrease the time required for diagnosis. The objective of this study was to determine the influence of serum sample inactivation on the performance of the latex agglutination test (LAT) for detecting antibodies against Paracoccidioides brasiliensis check for this species in other resources . The sensitivity of LAT from inactivated or non-inactivated samples was 73% and 83%, respectively and the LAT selectivity was 79% and 90%, respectively. The LAT evaluated here was no more specific than the double-immunodiffusion assay. We suggest the investigation of other methods for improving the LAT, such as the use of deglycosylated antigen.

Keywords
paracoccidioidomycosis - serodiagnosis - latex agglutination test - inactivation of samples

 
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