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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 107, No. 5, 2012, pp. 687-689
Bioline Code: oc12097
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 107, No. 5, 2012, pp. 687-689

 en The first report of the qnrB19, qnrS1 and aac(6’)-Ib-cr genes in urinary isolates of ciprofloxacin-resistant Escherichia coli check for this species in other resources in Brazil
Paiva, Magna Cristina; Nascimento, Andréa Maria Amaral; Camargo, Ilana Lopes Baratella Cunha; Lima-Bittencourt, Cláudia Iracema & Nardi, Regina Maria Drummond

Abstract

In this study, we investigated the presence of plasmid-mediated quinolone resistance (PMQR) genes among 101 ciprofloxacin-resistant urinary Escherichia coli check for this species in other resources isolates and searched for mutations in the quinolone-resistance-determining regions (QRDRs) of the DNA gyrase and topoisomerase IV genes in PMQR-carrying isolates. Eight isolates harboured the qnr and aac(6’)-Ib-cr genes (3 qnrS1, 1 qnrB19 and 4 aac(6’)-Ib-cr). A mutational analysis of the QRDRs in qnr and aac(6’)-Ib-cr-positive isolates revealed mutations in gyrA, parC and parE that might be associated with high levels of resistance to quinolones. No mutation was detected in gyrB. Rare gyrA, parC and parE mutations were detected outside of the QRDRs. This is the first report of qnrB19, qnrS1 and aac(6’)-Ib-cr-carrying E. coli isolates in Brazil.

Keywords
qnrS1 - qnrB19 - aac(6’)-Ib-cr

 
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