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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 107, No. 6, 2012, pp. 713-719
Bioline Code: oc12111
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 107, No. 6, 2012, pp. 713-719

 en Trypanosoma rangeli check for this species in other resources protein tyrosine phosphatase is associated with the parasite’s flagellum
Prestes, Elisa Beatriz; Bayer-Santos, Ethel; Stoco, Patrícia Hermes; Sincero, Thaís Cristine Marques; Wagner, Glauber; Umaki, Adriana; Fragoso, Stenio Perdigão; Bordignon, Juliano; Steindel, Mário & Grisard, Edmundo Carlos

Abstract

Protein tyrosine phosphatases (PTPs) play an essential role in the regulation of cell differentiation in pathogenic trypanosomatids. In this study, we describe a PTP expressed by the non-pathogenic protozoan Trypanosoma rangeli check for this species in other resources (TrPTP2). The gene for this PTP is orthologous to the T. brucei TbPTP1 and Trypanosoma cruzi check for this species in other resources (TcPTP2) genes. Cloning and expression of the TrPTP2 and TcPTP2 proteins allowed anti-PTP2 monoclonal antibodies to be generated in BALB/c mice. When expressed by T. rangeli epimastigotes and trypomastigotes, native TrPTP2 is detected as a ~65 kDa protein associated with the parasite’s flagellum. Given that the flagellum is an important structure for cell differentiation in trypanosomatids, the presence of a protein responsible for tyrosine dephosphorylation in the T. rangeli flagellum could represent an interesting mechanism of regulation in this structure.

Keywords
Trypanosoma rangeli - protein tyrosine phosphatase - flagellum

 
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